| 质粒名称: | pGL3-(2 BsaI)-PGK-puroR |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002171 |
| 产品用途: | Basic vector containing two BsaI restriction sites. |
| 存储实验室: | Zhuobin Liang Lab 梁卓斌课题组 |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | PGK |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | 未知 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
TRU-PE: A Universal, Trackable Prime Editor Toolkit for Robust Single-and Multi-Locus Genome Engineering
Zhichao Qiu, Keke Sun, Qingwei Zeng, Ziwei Luo, Xinran Liu, Yaping Li, Xiang Lei, Ruilin Zhao, Zhen Zhang, Damia Romero-Moya, Yuan Yang, Xuan Wang, Ehsan Hashemi, Joe Z. Zhang, Xiaolong Wang, Alessandra Giorgetti, Zhuobin Liang
bioRxiv 2026.02.20.706938
|
pGL3-(2 BsaI)-PGK-puroR
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pGL3-(2 BsaI)-PGK-puroR载体质粒基本信息
pGL3-(2 BsaI)-PGK-puroR质粒图谱载体图谱和pGL3-(2 BsaI)-PGK-puroR载体序列质粒序列多克隆位点信息
-PGK-puroR载体图谱.png)
pGL3-(2 BsaI)-PGK-puroR质粒载体简介
pGL3-(2 BsaI)-PGK-puroR质粒序列载体序列
LOCUS pGL3-(2 BsaI)-PGK-puroR 4591 bp DNA circular SYN 06-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4591)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 19, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4591
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(21..48)
/label=OL459
primer_bind 37..61
/label=OL441
primer_bind complement(41..65)
/label=OL442
primer_bind 55..81
/label=OL458
misc_feature 100..217
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 266..776
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 695..713
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 798..1397
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(798..817)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 1294..1314
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
polyA_signal 1544..1665
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1555..1574)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 1609..1628
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(1913..1930)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2084..2672)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2164..2183)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(2843..3703)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3466..3485
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(3704..3808)
/gene="bla"
/label=AmpR promoter
rep_origin 3835..4290
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3922..3941)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4132..4153
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 4421..4469
/note="synthetic polyadenylation signal"
misc_feature 4483..4574
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 4523..4542
/label=RVprimer3
/note="pGL3 vector, forward primer"
ORIGIN
1 agtgaacgga tctcgacggt atcgatcacg agactagcct tgagaccgag agagggtctc
61 attctcgacc tcgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
121 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
181 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
241 agatccactt tggccgcggc tcgagggggt tggggttgcg ccttttccaa ggcagccctg
301 ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc
361 ctgggactcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc
421 ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt
481 tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg
541 gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct
601 gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg
661 gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg
721 agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg
781 atccaccgga gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
841 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
901 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
961 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
1021 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
1081 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
1141 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
1201 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
1261 gggtgcccgc cttcctggaa acctccgcgc cccgcaacct ccccttctac gagcggctcg
1321 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
1381 gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac
1441 gaccccatgc atcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
1501 actttctaga gtcggggcgg ccggccgctt cgagcagaca tgataagata cattgatgag
1561 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
1621 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
1681 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
1741 ctctacaaat gtggtaaaat cgataaggat ccgtcgaccg atgcccttga gagccttcaa
1801 cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt
1861 cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct tcctcgctca
1921 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
1981 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
2041 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
2101 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
2161 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
2221 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
2281 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
2341 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
2401 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
2461 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
2521 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
2581 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
2641 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
2701 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
2761 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
2821 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
2881 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
2941 gggagggctt accatctggc cccagtgctg caatgatacc gcgggaccca cgctcaccgg
3001 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
3061 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
3121 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
3181 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
3241 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
3301 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
3361 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
3421 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
3481 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
3541 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
3601 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
3661 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
3721 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
3781 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc
3841 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
3901 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
3961 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
4021 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
4081 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
4141 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
4201 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
4261 attttaacaa aatattaacg cttacaattt gccattcgcc attcaggctg cgcaactgtt
4321 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gcccaagcta ccatgataag
4381 taagtaatat taaggtacgg gaggtacttg gagcggccgc aataaaatat ctttattttc
4441 attacatctg tgtgttggtt ttttgtgtga atcgatagta ctaacatacg ctctccatca
4501 aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc
4561 cagaacattt ctctatcgat aggtaccgat t
//
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pGL3-(2 BsaI)-PGK-puroR质粒载体应用举例
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